Autoantibodies against Antigens of the Skin
Indirect Immunofluorescence Test: Dermatology Mosaic 9
- Oesophagus: antibodies against epidermal basement membrane (top left). Desmoglein 1 + 3 (middle left, bottom left). BP230 gC (top right). BP230 whole length (middle right). BP180 (NC16A-4X) (bottom right).
- Screening and differentiation test for detection of skin-specific antibodies.
- Indication: autoimmune bullous dermatoses.
- Initial dilution 1 : 10; conjugate class anti-human IgG, FITC-labelled.
- The BIOCHIP Mosaic consists of 6 substrates:primate oesophagus,desmoglein 1-expressing cells, desmoglein 3-expressing cells, BP230-expressing cells (whole length), BP230-expressing cells (gC) and BP180 (EUROPLUS). Thus a comprehensive antigen spectrum is available in a single analysis, allowing targeted serological diagnosis.
- Autoantibodies against intercellular target structures (prickle cell desmosomes) can be reliably detected using tissue sections of oesophagus and tongue, although with this combination it is difficult to distinguish between desmoglein 1 and desmoglein 3. When specific transfected cells are employed in addition, a targeted diagnosis in a single test run is possible.
- Antibodies against prickle cell desmosomes Antibodies against prickle cell desmosomes react with surface antigens of keratinocytes. Tissue sections of oesophagus and tongue show a granular fluorescence in the intercellular matter in the whole stratum spinosum.
- When autoantibodies against BP180 or BP230 are present, the epidermal basement membrane in the oesophagus or tongue is visible as a fine linear colouring between the stratum basale and the connective tissue. These antibodies can be differentiated by means of BP180-NC16A-4X coated BIOCHIPS and cells transfected with BP230 (whole length or globular C-terminal domain (gC), respectively.
- This BIOCHIP Mosaic can be customised with further substrates if required, e.g. tongue (antibodies against prickle cell desmosomes, epidermal basement membrane), bladder (antibodies against plakins), salt split skin (antibodies against epidermal basement membrane).
| Antigen |
| Order No. |
Desmoglein 1 (transfected/non-transfected cells) | FA 1495-####-50 G | ||
Desmoglein 3 (transfected/non-transfected cells) | FA 1496-####-50 G | ||
Oesophagus | FA 1501-#### G | ||
Oesophagus/Tongue | FA 1501-####-1 G | ||
Tongue | FA 1502-#### G | ||
Bladder mucosa | FA 1507-#### G | ||
Split skin | FA 150b-#### G |
Microplate ELISA: Anti-Desmoglein 1, Anti-Desmoglein 3, Anti-BP180-NC16A-4X, Anti-BP230-CF
- Incubated ELISA Anti-Desmoglein 1 and 3, Anti-BP180-NC16A-4X, Anti-BP230-CF.
- Monospecific detection of antibodies against desmoglein 1, desmoglein 3, BP180 und BP230.
- Indication: autoimmune bullous dermatoses.
- Serum dilution 1 : 100; conjugate class anti-human IgG, POD-labelled.
- 3-point calibration, quantitative.
- Identical incubation conditions and times: all tests can be combined on one microplate.
- Recombinant antigens: extracellular domain of desmoglein 1 or 3, tetramer of NC16A domain of BP180 protein, C-terminal segment of BP230 protein. The corresponding human cDNA is produced in E. coli (BP180-NC16A-4X, BP230-CF) or in mammalian cells (desmoglein 1, desmoglein 3).
