Autoantibodies against Mitochondria (AMA)
Indirect Immunofluorescence Test: EUROPLUS™ Rat Kidney and M2-3E BIOCHIPs
- Rat kidney and M2-3E BIOCHIP: antibodies against mitochondria (AMA).
- Screening test for the detection of antibodies against mitochondria (AMA) including simultaneous confirmation of the subtype AMA M2.
- Indication: primary biliary cirrhosis (PBC).
- Initial dilution 1 : 100; polyvalent conjugate anti-human IgAGM, FITC-labelled.
- Rat kidney is the standard substrate for detecting anti-mitochondrial antibodies. Nine AMA types (M1 to M9) can be differentiated.
- The BIOCHIP coated with M2-3E permits monospecific confirmation of antibodies against the native pyruvate dehydrogenase complex and the recombinant M2 fusion protein (BPO) in one single test procedure, thus a PBC can be diagnosed serologically with confidence.
- This BIOCHIP Mosaic™ can be supplemented as required using additional substrates, e.g. HEp-2 cells (ANA, nuclear dots), rat liver (liver-kidney microsomes, LKM) or rat stomach (ASMA).
EUROASSAY: AMA Profile M2, M4, M9
- Incubated EUROASSAY AMA Profile M2, M4, M9.
- Differentiation of mitochondrial antibodies (AMA).
- Indication: primary biliary cirrhosis (PBC).
- Serum dilution 1 : 100; conjugate class anti-human IgGM, AP-labelled.
- 3 relevant mitochondrial antibodies can be detected simultaneously and monospecifically: antibodies against M2, M4, M9.
- Native antigens: pyruvate dehydrogenase complex (M2), sulfite oxidase (M4), glycogen phosphorylase (M9).
| Anti- |
| Associated diseases |
M2 | Primary biliary cirrhosis (high titers), other chronic liver diseases | ||
M4 | Primary biliary cirrhosis | ||
M9 | Early phase of primary biliary cirrhosis |
Microplate ELISA: Anti-M2-3E
- Incubated ELISA anti-M2-3E.
- Differentiation of mitochondrial antibodies (AMA).
- Indication: primary biliary cirrhosis (PBC).
- Serum dilution 1 : 100; conjugate class anti-human IgG, POD-labelled.
- Antibodies against M2 can be determined quantitatively in RU/ml.
- Antigen: native pyruvate dehydrogenase complex plus recombinant M2 fusion protein (BPO) containing the immunogenic domains of the E2 subunits of PDH, BCOADH and OGDH.
